Pacific blue fluorophore.

Welcome to FluoroFinder’s Reagent Directory Directory. Here you can find informational pages on fluorescent dyes in our system, with information on excitation and emission characteristics, optimal laser and filter sets, common applications, and more. We are adding dyes to the directory each day so check back for the latest updates. An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin, and yet not recognized by the latter’s ligase (W37V LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, E20G/W37T LplA, that ligates …The VioGreen™ Dye is a fluorochrome with a large Stokes shift, emitting strong fluorescence at 520 nm upon excitation at 405 nm. It is a non-protein fluorochrome with significantly increased mean fluorescence intensities and higher stain indices compared to related fluorochromes such as Pacific Orange™, Krome Orange™, and BD Horizon V500.Dec 19, 2016 · We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach potentially applicable to a wide range of ... An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, (E20G/W37T)LplA, that ligates …

Spectrum [Pacific Blue] Pacific Blue is a fluorescent compound with an excitation peak at 404 nm and an emission peak at 455 nm. Other spectra of interest include: iFluor 405, iFluor 440, and iFluor 430. Pacific Blue belongs to the following categories: Amine Reactive Dyes and Probes for Conjugation. Purchase Pacific Blue here.Not for use in diagnostic or therapeutic procedures. APC-Cy7: US patent 5,714,386. Alexa Fluor® is a registered trademark, and Pacific Blue™ is a trademark of ...

10 янв. 2022 г. ... BFP (Blue Fluorescent Protein), 380 nm, 439 nm. Alexa 350, 343 nm, 441 nm ... Pacific Blue antibody conjugate pH 8.0, 404 nm, 455 nm. PO-PRO-1 ...

In the example, if the target fluorophore is the Alexa Fluor 488, using the Blue Laser and the GFP channel, 52.8% of the Alexa Fluor 488 emission spectra is captured. By reviewing the table horizontally and using the Blue Laser and the GFP channel or emission filter, the fluorescence emission from the GFP fluorophore is also captured with Alexa ...For comparison, Pacific Blue (PB), a single molecule dye with near maximum absorption at 405 nm, has an extinction coefficient of 4.6 × 10 4 M −1 cm −1, a quantum efficiency of 78%, and a longer lifetime of 9 ns. Practically, the brightness of a fluorochrome is proportional to the extinction coefficient and quantum efficiency, and ...Note that the standard Dapi/Hoechst filter set will not work with fluorophores that excite at 405 nm. For instance, Alexa 405 and Pacific Blue are bad ...Fluorochrome selection is a key step in designing multi-color antibody panels. The list of available fluorochromes is continuously growing, fitting current needs in clinical flow cytometry to simultaneously use more markers to better define multiple leukocyte subpopulations in a single tube. Several criteria guide fluorochrome selection: i) the ...

Multi-color flow cytometry and cell sorting applications require the use of a wide range of fluorochrome-conjugated antibodies and dyes. MACSQuant X, MACSQuant Analyzer 10, and MACSQuant Tyto® compatible dyes ... Violet 450 AM, Indo-1, Cascade Blue®, DAPI, Vybrant® DyeCycle™ Violet, eBFP, CFP, eFluor® 450, Super Bright 436, Pacific Blue ...

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An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter’s ligase ([superscript W37V]LplA) or any tryptophan 37 point mutant. Here we report the results of a structure-guided, two-residue screening matrix to discover an LplA double mutant, [superscript E20G/W37T]LplA, that ...Alexa Fluor 647 dye. Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines. For stable signal generation in imaging and flow cytometry, Alexa Fluor 647 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high ...If you’re in the market for a new or used boat, one valuable tool that can help you negotiate the best price is a blue book for boats. Before diving into negotiating a price using a blue book for boats, it’s important to have a good underst...Fluorophore Selection Guide. Fluorophores enable researchers to expand the limits of their multicolor experiments in high content analysis, microscopy, and flow cytometry platforms. Our fluorescent dyes are offered conjugated to antibodies, streptavidin, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling ...Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. …Dec 1, 2019 · Fluorochrome selection is a key step in designing multi-color antibody panels. The list of available fluorochromes is continuously growing, fitting current needs in clinical flow cytometry to simultaneously use more markers to better define multiple leukocyte subpopulations in a single tube. Several criteria guide fluorochrome selection: i) the ...

Blue (488 nm) BD Horizon™ BB515 BD Horizon™ PE-CF594 PE-Cy™5 PE PE-Cy™7 FITC Alexa Fluor® 488 PerCP-Cy™5.5 PerCP Yellow/Green (561 nm) PE BD Horizon PE-CF594 ... Many factors can influence the relative fluorochrome/reagent performance on a given instrument, including laser power, PMT voltage, optical filters, ant ibody clone, and ...Pacific Blue: 455: 500–600: Pacific Green: 500 : Horizon V500, Brilliant Violet 510, VioGreen, Brilliant Violet 570: eFluor 506: 510: Pacific Orange: 550: 600–700: Super Bright 600: 600 : Brilliant Violet 605, ... Fluorophore staining index …The traditional blue-green 488 nm laser line produced by argon-ion sources was and remains (via newer solid state sources) the primary laser line for most flow cytometric analysis. The 488 nm line excites fluorescein, a low molecular weight fluorochrome that forms the molecular basis for a wide variety of fluorescent cell tags and physiological ...An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant.Super Bright 436 Dye. Invitrogen eBioscience Super Bright 436 dye is a polymer-based fluorophore excited by the violet laser (405 nm) that emits signal at 436 nm with high efficacy. The technology behind the Super Bright polymer dye family makes them useful for identifying low-abundance markers on a cell surface in flow cytometry. The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining or prior to elimination of biohazardous materials using formaldehyde fixation. This kit has been optimized and validated for use with a violet laser flow cytometer ...

The LIVE/DEAD Fixable Red (615) Viability kit for 488 and 561 nm excitation was used to differentially stain a mixture of live (left peak) and heat-treated Jurkat cells (right peak). Cells in (A) were not fixed; cells in (B) were fixed in 3.7% formaldehyde following staining. Samples were analyzed by flow cytometry using 488 nm excitation and ...

BD Horizon Brilliant™ Blue 700 (BB700) (Ex max 485 nm/Em max 693 nm) is a dye that was exclusively developed by BD Life Sciences as brighter alternative to PerCP-Cy5.5. This dye also has less cross-laser excitation off the 405-nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.Two of the most commonly used fluorophores in flow cytometry, R-phycoerythrin (R-PE) and allophycocyanin (APC), are phycobiliproteins originally derived from algae. Both proteins contain fluorescent phycobilin chromophores embedded into a protein scaffolding which accounts for their large size.from that of commonly used green fluorophores. Alexa Fluor 405: Excited by 405nm violet laser. Alexa Fluor 405 is an extremely dim dye, for that reason it should be only used for the brightest antigens. It is a derivated of the Cascade Blue dye. Alexa Fluor 405 conjugates show minimal spectral overlap with green fluorophores andAlexa Fluor 488 Dye. Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range. We offer Alexa Fluor 488 dye conjugated to a variety of antibodies, peptides ...16 авг. 2005 г. ... If calculations indicate that the protein conjugate is labeled with significantly more than seven moles of fluorophore per mole of IgG antibody, ...eFluor 450 dye is a small-molecule fluorescent dye with spectral properties similar to Pacific Blue dye. It was developed to exhibit equal or better fluorescence intensity when compared to Horizon V500 dye or Pacific Blue dye in multicolor applications. Fluorescence intensity comparison. Human PBMCs stained with Anti-CD45RA ( clone HI100 ...One of the most interesting examples is Pacific Blue (PB) 7, a fluorophore that differs from HC only in the two fluorine atoms at C6 and C8 of the coumarin ring …Marina Blue and Pacific Blue dyes, both of which are based on the 6,8-difluoro-7-hydroxycoumarin fluorophore, exhibit bright blue fluorescence emission near 460 nm (Amine-reactive, ultraviolet light-excitable fluorophores for labeling proteins and nucleic acids—Table 1.12).We report a new method to quantify the affinity of small molecules for proteins. This method is based on Förster resonance energy transfer (FRET) between endogenous tryptophan (Trp) residues and the coumarin-derived fluorophore Pacific Blue (PB). Tryptophan residues are frequently found in proteins near ligand-binding sites, making this approach …Spark Blue™ 550. Spark Blue™ 550 provides a unique option for adding an additional fluorophore to a high-parameter (>22 color) multicolor panel on spectral unmixing cytometers like the Cytek™ Aurora or SONY ID7000™ Spectral Cell Analyzer. It is excited off of the 488 nm laser and emits at 540 nm, filling the spectral gap between ...

Structure-guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells 2011 Sep 27;50 (38):8221-5. doi: …

BV421, V450, Pacific Blue,. Alexa405, eFluor450. BFP. DAPI,. SYTOX Blue. eFluor780,. LD405. Prolifn dye (eFluor, V450). FL10. -‐-‐. 525/40 505 -‐ 545. Krome ...

The Fluorophore Brightness Index Score is a relative indication of fluorescence intensity above the background for each fluorophore antibody conjugation (1=dim, 5=brightest). These values can differ depending upon the flow cytometer, the instrument filters and settings, laser power, the antibody clone and the antigen target, f/p ratios, the ... Quantification of Small Molecule–Protein Interactions using FRET between Tryptophan and the Pacific Blue Fluorophore. ACS Omega 2016, 1 (6) , 1266-1276. DOI: 10.1021/acsomega.6b00356. Vlad Popa, Danielle A. Trecroce, …An example is the blue fluorophore Pacific Blue, which is isosteric to 7-hydroxycoumarin and yet not recognized by the latter's ligase ((W37V)LplA) or any tryptophan 37 point mutant.545 nm) fluorochrome is part of the BD family of blue (488-nm) laser dyes. Driven by BD innovation, RB545 can be used on spectral cytometers and is designed to be excited by the blue (488-nm) laser with minimal excitation by the 561-nm yellow-green laser. RB545 has minimal spillover into yellow-green detectors and a brightness level similar to ...Our Fluorophore Equivalency Tool will help you find BioLegend’s equivalent fluorophores compared to other commercially available fluorophores. Simply select the name of a competitor's fluorophore from the dropdown menu to see BioLegend's equivalents. The tool also allows you to preview the excitation and emission spectra of our fluorophores ...Try the new and improved version of Full Spectrum Viewer in Cytek® Cloud! Sign Up For A Free Account. The new Full Spectrum Viewer features: More fluorescent tags to select from. Fluorescent tag signatures. Complexity™ Index. Similarity™ Index. Stain Index Reduction. Transfer fluorescent tags to experiment templates.Fluorophore at a Glance: An upgrade over FITC in terms of brightness. A bright and spectrally clean fluorophore that only exhibits significant spillover into Spark Blue™ 550. Few, if any, fluors spill into KIRAVIA Blue 520™. Utilize this fluor on ubiquitously expressed markers or markers with varying levels of expression.Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Pacific Green dye is a bright green-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 411/510 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 ...

brightest fluorochromes offered by BD Life Sciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to similar excitation and emission properties, BD Horizon ™ BV421 and BD Horizon V450 cannot be used simultaneously. • • • • • • 405 nm 450/40Invitrogen Molecular Probes Pacific Blue dye is a bright blue-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 410/455 nm. Conjugates of this dye are strongly fluorescent even at neutral pH. Invitrogen products such as Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be ...Pacific Orange and Pacific Blue antibody labeling kits enable attachment of a violet light-excitable fluorescent label on antibodies (up to 100 µg per reaction). Each kit contains enough dye for five reactions. Product Overview Documents Showing 2 of 2 Save to list Add to cart Recurring order eligibleInstagram:https://instagram. claire halltransexual massage los angeles3 bedroom house for rent near me cheapjalen wilson injury Live/Dead Blue or DAPI excited off the UV laser. For cell surface staining, BV421™ is significantly brighter than other spectrally equivalent fluorophores including Pacific Blue™, BD Horizon™ V450, eFluor® 450, and Alexa Fluor® 405. It has an extinction coefficient of 2,500,000 M-1cm-1 at 405 nm, and an aqueous solution quantum 2006 troy bilt super bronconknews Pacific Blue™ RPA-T4 440/40 22.5 AmCyan RPA-T4 525/50 20.2 1,2,3 Fluorochromes listed with the same superscript number are read in the same detector, and thus would not normally be used in combination. Table 2. Common choices for 6-, 8-, and 10-color experiments. 6-color 8-color 10-color FITC or Alexa Fluor® 488 FITC or Alexa Fluor® … tcu ku basketball Vio Dye Fluorophores. Vio® and Vio® Bright Dyes represent a family of fluorochromes for flow cytometry and fluorescence microscopy. They are characterized by high fluorescence intensities and low spillover, making them an ideal choice for multicolor applications. Combined with traditional fluorochromes, such as FITC, PE, PerCP, and APC, they ...* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.The LIVE/DEAD Fixable Red (615) Viability kit for 488 and 561 nm excitation was used to differentially stain a mixture of live (left peak) and heat-treated Jurkat cells (right peak). Cells in (A) were not fixed; cells in (B) were fixed in 3.7% formaldehyde following staining. Samples were analyzed by flow cytometry using 488 nm excitation and ...